Wang, K., Wang, S., Chen, Y. et al. Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain. Cell Res (2021). https://doi.org/10.1038/s41422-021-00479-9
Human protein atlas とても使いやすく、勉強になる。半日くらいパラパラ見ていて飽きない。
Spared nerve injury
神経枝結紮損傷モデル 坐骨神経の一部のみを結紮する疼痛モデル
ATF3 Activating transcription factor 3
Neuronal Damageを起こした神経細胞で発現する。
ATF3の発現が見られた細胞において、同じように発現がUpregulateされているmRNA
（補足は上記サイトから）
CLDN9
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity
Mrgprd
May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i)
Trappc3l
May play a role in vesicular transport from endoplasmic reticulum to Golgi
GAL
Endocrine hormone of the central and peripheral nervous systems that binds and activates the G protein-coupled receptors GALR1, GALR2, and GALR3. This small neuropeptide may regulate diverse physiologic functions including contraction of smooth muscle of the gastrointestinal and genitourinary tract, growth hormone and insulin release and adrenal secretion.
Gfra3
Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase
CLCF1
In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
smartSeq2 technology:
scRNA sequencing method の１つ
Nature protocol
https://www.nature.com/articles/nprot.2014.006
